65. Which of the following statements regarding Michaelis-Menten kinetic analyses of enzyme action are correct?① The total enzyme concentration studied at each substrate concentration is fixed in analysis of enzyme kinetics.② Formation of enzyme-substrate complex does not appreciably decrease the concentration of substrate.③ cat reduces with competitive inhibition.④ Maximal velocity is reached when the enzyme-substrate complex is equal to the total concentration of enzyme present.⑤ The initial reaction velocity should be measured because most of the substrate has not been converted to product.
(A) ①②③④
(B) ①②③⑤
(C) ①②④⑤
(D) ②③④⑤
(E) ①③④⑤