50. A circular 3518 bp plasmid DNA has EcoRI restriction sites at position 86 and 1435; PstI sites at position 1108 and 2950;and a BamHI site at position 2623. Which one of the following choices is not correct?
(A) EcoRI digestion yields two bands in agarose gel.
(B) When digestion with EcoRI and BamHI, three bands will be observed in agarose gel.
(C) After digestion with EcoRI, PstI and and BamHI, five bands will be observed in agarose gel.
(D) When digestion with EcoRV and PstI, only two bands will be observed in agarose gel.
(E) BamHI digestion yields only one band in agarose gel.